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Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,3',6'-dihydroxy- cas no:2321-07-5

Synonyms: Fluorescein(8CI);3,6-Dihydroxyspiro[xanthene-9,3'-phthalide];3',6'-Fluorandiol;Benzoic acid,2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-;C.I. 45350:1;D and C Yellow No. 7;3',6'-Dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one;C Yellow No. 7;Japan Yellow 201;Japan Yellow No. 201;Resorcinolphthalein;Yellow fluorescein;

Identification
NameSpiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,3',6'-dihydroxy-
CAS2321-07-5
SynonymsFluorescein(8CI);3,6-Dihydroxyspiro[xanthene-9,3'-phthalide];3',6'-Fluorandiol;Benzoic acid,2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-;C.I. 45350:1;D and C Yellow No. 7;3',6'-Dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one;C Yellow No. 7;Japan Yellow 201;Japan Yellow No. 201;Resorcinolphthalein;Yellow fluorescein;
EINECS(EC#)219-031-8
Molecular FormulaC20H12O5
Molecular Weight332.31
Chemical Properties
Appearanceorange-red crystalline powder
refractive index1.46355
storage temp−20°C
Globally Harmonized System of Classification adn Labelling of Chemicals(GHS)
Hazardclass3
Safety Data

Hazard Xi:Irritant;
Risk R36;R43
Safety

Hazard Codes of Fluorescein (CAS NO.2321-07-5): IrritantXi
Risk Statements: 43-36/37/38 
R43: May cause sensitization by skin contact. 
R36/37/38: Irritating to eyes, respiratory system and skin.
Safety Statements: 22-24/25-26-36/37/39-27 
S22: Do not breathe dust. 
S24/25: Avoid contact with skin and eyes. 
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. 
S36/37/39: Wear suitable protective clothing, gloves and eye/face protection. 
S27: Take off immediately all contaminated clothing.
WGK Germany: 3
RTECS: LM5075000
F: 10-21
Poison by intravenous route. Moderately toxic by intraperitoneal route. Mutation data reported. When heated to decomposition it emits acrid smoke and irritating fumes. See also FLUORESCEIN SODIUM.


Toxicity

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
guinea pig LDLo unreported 400mg/kg (400mg/kg)   "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1289, 1935.
man TDLo intravenous 7143nL/kg (7143mL/kg) GASTROINTESTINAL: NAUSEA OR VOMITING

SKIN AND APPENDAGES (SKIN): "DERMATITIS, OTHER: AFTER SYSTEMIC EXPOSURE"
American Journal of Ophthalmology. Vol. 126, Pg. 837, 1998.
mouse LD50 intravenous 300mg/kg (300mg/kg)   United States Patent Document. Vol. #4775625,
mouse LDLo unreported 600mg/kg (600mg/kg)   "Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1289, 1935.
rabbit LDLo intravenous 300mg/kg (300mg/kg)   International Journal of Leprosy and Other Mycobacterial Diseases. Vol. 2, Pg. 257, 1934.
rat LDLo intraperitoneal 600mg/kg (600mg/kg)   International Journal of Leprosy and Other Mycobacterial Diseases. Vol. 2, Pg. 257, 1934.

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